The Nlrp6 inflammasome is not required for baseline colonic inner mucus layer formation or function.

ABSTRACT: The inner mucus layer (IML) is a critical barrier that protects the colonic epithelium from luminal threats and inflammatory bowel disease. Innate immune signaling is thought to regulate IML formation via goblet cell Nlrp6 inflammasome activity that controls secretion of the mucus structural component Muc2. We report that isolated colonic goblet cells express components of several inflammasomes; however, analysis of IML properties in multiple inflammasome-deficient mice, including littermate-controlled Nlrp6^-/- , detect a functional IML barrier in all strains. Analysis of mice lacking inflammasome substrate cytokines identifies a defective IML in Il18^-/- mice, but this phenotype is ultimately traced to a microbiota-driven, Il18-independent effect. Analys

SUBMITTER: Volk JK 

PROVIDER: S-EPMC6829596 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature