SGK regulates pH increase and cyclin B-Cdk1 activation to resume meiosis in starfish ovarian oocytes.

ABSTRACT: Tight regulation of intracellular pH (pH_i) is essential for biological processes. Fully grown oocytes, having a large nucleus called the germinal vesicle, arrest at meiotic prophase I. Upon hormonal stimulus, oocytes resume meiosis to become fertilizable. At this time, the pH_i increases via Na⁺/H⁺ exchanger activity, although the regulation and function of this change remain obscure. Here, we show that in starfish oocytes, serum- and glucocorticoid-regulated kinase (SGK) is activated via PI3K/TORC2/PDK1 signaling after hormonal stimulus and that SGK is required for this pH_i increase and cyclin B-Cdk1 activation. When we clamped the pH_i at 6.7, corresponding to the pH_i of unstimulated ovarian oocytes, hormonal stim

SUBMITTER: Hosoda E 

PROVIDER: S-EPMC6829648 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature