An intrinsic compartmentalization code for peripheral membrane proteins in photoreceptor neurons.

ABSTRACT: In neurons, peripheral membrane proteins are enriched in subcellular compartments, where they play key roles, including transducing and transmitting information. However, little is known about the mechanisms underlying their compartmentalization. To explore the roles of hydrophobic and electrostatic interactions, we engineered probes consisting of lipidation motifs attached to fluorescent proteins by variously charged linkers and expressed them in Xenopus rod photoreceptors. Quantitative live cell imaging showed dramatic differences in distributions and dynamics of the probes, including presynapse and ciliary OS enrichment, depending on lipid moiety and protein surface charge. Opposing extant models of ciliary enrichment, most probes were weakly membrane bound and diffused through t

SUBMITTER: Maza NA 

PROVIDER: S-EPMC6829649 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature