New factors for protein transport identified by a genome-wide CRISPRi screen in mammalian cells.

ABSTRACT: Protein and membrane trafficking pathways are critical for cell and tissue homeostasis. Traditional genetic and biochemical approaches have shed light on basic principles underlying these processes. However, the list of factors required for secretory pathway function remains incomplete, and mechanisms involved in their adaptation poorly understood. Here, we present a powerful strategy based on a pooled genome-wide CRISPRi screen that allowed the identification of new factors involved in protein transport. Two newly identified factors, TTC17 and CCDC157, localized along the secretory pathway and were found to interact with resident proteins of ER-Golgi membranes. In addition, we uncovered that upon TTC17 knockdown, the polarized organization of Golgi cisternae was altered, creating glycosyl

SUBMITTER: Bassaganyas L 

PROVIDER: S-EPMC6829651 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature