Seipin negatively regulates sphingolipid production at the ER-LD contact site.

ABSTRACT: Seipin is known for its critical role in controlling lipid droplet (LD) assembly at the LD-forming subdomain of the endoplasmic reticulum (ER). Here, we identified a new function of seipin as a negative regulator for sphingolipid production. We show that yeast cells lacking seipin displayed altered sensitivity to sphingolipid inhibitors, accumulated sphingoid precursors and intermediates, and increased serine palmitoyltransferase (SPT) and fatty acid (FA) elongase activities. Seipin associated with SPT and FA elongase, and the interaction was reduced by inhibitors for sphingolipid synthesis in a concentration-dependent manner. We further show that the interactions of seipin with SPT and FA elongase occurred at ER-LD contacts and were likely regulated differentially. Further evidence indica

SUBMITTER: Su WC 

PROVIDER: S-EPMC6829658 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature