Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium.

ABSTRACT:

Background

Serum-free xenogen-free defined media and continuous controlled physiological cell culture conditions have been developed for stem cell therapeutics, but the effect of these conditions on the relative potency of the cell product is unknown. As such, we conducted a head-to-head comparison of cell culture conditions on human heart explant-derived cells using established in vitro measures of cell potency and in vivo functional repair.

Methods

Heart explant-derived cells cultured from human atrial or ventricular biopsies within a serum-free xenogen-free media and a continuous physiological culture environment were compared to cells cultured under traditional (high serum) cell culture conditions in a standard clean room facility.

Results

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SUBMITTER: Mount S 

PROVIDER: S-EPMC6829847 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature