A comparative study of single-particle cryo-EM with liquid-nitrogen and liquid-helium cooling.

ABSTRACT: Radiation damage is the most fundamental limitation for achieving high resolution in electron cryo-microscopy (cryo-EM) of biological samples. The effects of radiation damage are reduced by liquid-helium cooling, although the use of liquid helium is more challenging than that of liquid nitrogen. To date, the benefits of liquid-nitrogen and liquid-helium cooling for single-particle cryo-EM have not been compared quantitatively. With recent technical and computational advances in cryo-EM image recording and processing, such a comparison now seems timely. This study aims to evaluate the relative merits of liquid-helium cooling in present-day single-particle analysis, taking advantage of direct electron detectors. Two data sets for recombinant mouse heavy-chain apoferritin cooled with liquid-n

SUBMITTER: Pfeil-Gardiner O 

PROVIDER: S-EPMC6830223 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature