Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance.

ABSTRACT: Small GTPases regulate cytoskeletal dynamics, cell motility, and division under precise spatiotemporal control. Different small GTPases exhibit cross talks to exert feedback response or to act in concert during signal transduction. However, whether and how specific cytoskeletal components' feedback to upstream signaling factors remains largely elusive. Here, we report an intriguing finding that disruption of the Arp2/3-branched actin specifically reduces RhoA activity but upregulates its total protein abundance. We further dissect the mechanisms underlying these circumstances and identify the altered cortactin/p190RhoGAP interaction and weakened CCM2/Smurf1 binding to be involved in GTP-RhoA reduction and total RhoA increase, respectively. Moreover, we find that cytokinesis defects induced

SUBMITTER: Huang Y 

PROVIDER: S-EPMC6830327 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature