Dietary fish oil supplementation alters liver gene expressions to protect against LPS-induced liver injury in weanling piglets.

ABSTRACT: Here, the potential mechanisms of the protective effects of fish oil against LPS-induced liver injury in a piglet model were investigated by using RNA sequencing. Twenty-four piglets were used in a 2 × 2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline, on d 19). All piglets were slaughtered at 4 h after challenge, and liver samples were collected. Fish oil improved liver morphology and reduced TNF-α, IL-1β and IL-6 productions after LPS challenge. RNA sequencing analysis showed fish oil had significant effect on the expressions of genes involved in immune response during LPS-induced inflammation. Selected gene expression changes were validated using quantitative RT-PCR. Fish oil reduced the expressions of pro-infl

SUBMITTER: Zhang J 

PROVIDER: S-EPMC6830890 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature