Kinome analyses of inflammatory responses to swine barn dust extract in human bronchial epithelial and monocyte cell lines.

ABSTRACT: Exacerbated inflammation upon persistent barn organic dust exposure is a key contributor to the pathogenesis of lung inflammation and lung function decline. Barn dust constituents and the mechanisms contributing to the exacerbated inflammation are not clearly known. We set out to understand the inflammatory effects of Swine Barn Dust Extracts (SBDE) on human lung epithelial (BEAS2B) and macrophage (THP-1 monocyte derived) cell lines on a kinome array to determine phosphorylation events in the inflammatory signaling pathways. Upon identifying events unique to SBDE or those induced by innate immune ligands in each cell line, we validated the signaling pathway activation by transcriptional analyses of downstream inflammatory cytokines. Our findings indicate that SBDE-mediated pro-inflammatory

SUBMITTER: Nath Neerukonda S 

PROVIDER: S-EPMC6830909 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature