Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections.

ABSTRACT: Because of the close interaction between tumors and the immune system, immunotherapies are nowadays considered as the most promising treatment against cancer. In order to define the diagnosis and the subsequent therapy, crucial information about the immune cells at the tumor site is needed. Indeed, different types or activation status of cells may be indicative for specific and personalized treatments. Here, we present a quantitative method to identify ten different immuno-markers in the same tumor cut section, thereby saving precious samples and enabling correlative analysis on several cell families and their activation status in a tumor microenvironment context. We designed and fabricated a microfluidic chip with optimal thermomechanical and optical properties for fast delivery of reagen

SUBMITTER: Migliozzi D 

PROVIDER: S-EPMC6831597 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature