Diversely positive-charged gold nanoparticles based biosensor: A label-free and sensitive tool for foodborne pathogen detection.

ABSTRACT: Gold nanoparticles (AuNPs)-based lateral flow strip (LFS) enables a quick screening of foodborne bacteria in point-of-care (POC) diagnostics but suffers from the cumbersome labeling of antibodies and poor sensitive performance. Here, we innovated a label-free immunoassay to detect pathogenic bacteria by introducing diversely positive charges functionalized AuNPs ((+) AuNPs). The (+) AuNPs can be loaded on negatively charged bacteria via electrostatic interaction, leading to a color labeling to target bacteria. Afterward, the (+) AuNPs-bacteria complex can be specifically captured by monoclonal antibody (McAb) immobilized on test (T)-line. Under optimum conditions, the proposed LFS exhibited a lowest detectable concentration of 10³ CFU/mL for Salmonella enteritidis (S.

SUBMITTER: Bu T 

PROVIDER: S-EPMC6831733 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature