Chromosomal Recombination Targets in Chlamydia Interspecies Lateral Gene Transfer.

ABSTRACT: Lateral gene transfer (LGT) among Chlamydia trachomatis strains is common, in both isolates generated in the laboratory and those examined directly from patients. In contrast, there are very few examples of recent acquisition of DNA by any Chlamydia spp. from any other species. Interspecies LGT in this system was analyzed using crosses of tetracycline (Tc)-resistant C. trachomatis L2/434 and chloramphenicol (Cam)-resistant C. muridarum VR-123. Parental C. muridarum strains were created using a plasmid-based Himar transposition system, which led to integration of the Cam^r marker randomly across the chromosome. Fragments encompassing 79% of the C. muridarum chromosome were introduced into a C. trachomatis background, with the total

SUBMITTER: Suchland RJ 

PROVIDER: S-EPMC6832074 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature