Monitoring and Determining Mitochondrial Network Parameters in Live Lung Cancer Cells.

ABSTRACT: Mitochondria are dynamic organelles that constantly fuse and divide, forming dynamic tubular networks. Abnormalities in mitochondrial dynamics and morphology are linked to diverse pathological states, including cancer. Thus, alterations in mitochondrial parameters could indicate early events of disease manifestation or progression. However, finding reliable and quantitative tools for monitoring mitochondria and determining the network parameters, particularly in live cells, has proven challenging. Here, we present a 2D confocal imaging-based approach that combines automatic mitochondrial morphology and dynamics analysis with fractal analysis in live small cell lung cancer (SCLC) cells. We chose SCLC cells as a test case since they typically have very little cytoplasm, but an abundance of s

SUBMITTER: Mirzapoiazova T 

PROVIDER: S-EPMC6832496 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature