Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection.

ABSTRACT: During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening around the viral replication compartments correlated with their expansion. We also observed a gradual tra

SUBMITTER: Aho V 

PROVIDER: S-EPMC6832731 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature