Integrated strain- and process design enable production of 220 g L^-1 itaconic acid with Ustilago maydis.

ABSTRACT:

Background

Itaconic acid is an unsaturated, dicarboxylic acid which finds a wide range of applications in the polymer industry and as a building block for fuels, solvents and pharmaceuticals. Currently, Aspergillus terreus is used for industrial production, with titers above 100 g L^-1 depending on the conditions. Besides A. terreus, Ustilago maydis is also a promising itaconic acid production host due to its yeast-like morphology. Recent strain engineering efforts significantly increased the yield, titer and rate of production.

Results

In this study, itaconate production by U. maydis was further increased by integrated strain- and process engineering. Next-generation itaconate hyper-producing strains were generated using CRISPR/Cas9 and

SUBMITTER: Hosseinpour Tehrani H 

PROVIDER: S-EPMC6833137 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature