PADI3 plays an antitumor role via the Hsp90/CKS1 pathway in colon cancer.

ABSTRACT:

Background

CKS1 is highly expressed in colon cancer tissues, and is essential for cancer cell proliferation. The downstream molecular mechanism of CKS1 has been fully studied, but the upstream regulatory mechanism of it is still unclear. Earlier research found that PADI3 plays its anti-tumor roles via suppress cell proliferation, in this study, we found that the expression pattern of PADI3 and CKS1 are negatively correlated in colon cancer tissues, and overexpression of PADI3 can partly reverse CKS1 induced cancer cell proliferation. However, the regulatory mechanism of PADI3 and CKS1 in the tumorigenesis of colon cancer is still unclear and need to do further research.

Methods

Western blot and real-time PCR were used to detect the expression levels of genes. CCK-8 and colon

SUBMITTER: Chai Z 

PROVIDER: S-EPMC6833139 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature