Investigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis.

ABSTRACT:

Background

Terpenoids are of high interest as chemical building blocks and pharmaceuticals. In microbes, terpenoids can be synthesized via the methylerythritol phosphate (MEP) or mevalonate (MVA) pathways. Although the MEP pathway has a higher theoretical yield, metabolic engineering has met with little success because the regulation of the pathway is poorly understood.

Results

We applied metabolic control analysis to the MEP pathway in Escherichia coli expressing a heterologous isoprene synthase gene (ispS). The expression of ispS led to the accumulation of isopentenyl pyrophosphate (IPP)/dimethylallyl pyrophosphate (DMAPP) and severely impaired bacterial growth, but the coexpression of ispS and isopentenyl diphosphate isomerase (idi) restored normal growth and wild-type IP

SUBMITTER: Volke DC 

PROVIDER: S-EPMC6833178 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature