High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis.

ABSTRACT:

Background

Bovine babesiosis is caused by protozoan parasites of the genus Babesia and presents a wide spectrum of clinical manifestations. Disease severity depends on the type of Babesia species infection. Generally, B. bovis and B. bigemina are considered as the causative agents of bovine babesiosis; in addition, Babesia ovata and B. major are a group of benign bovine piroplasms. Therefore, species identification is important for diagnosis, epidemiological investigations and follow-up management.

Methods

Real-time PCR combined with high resolution melting (RT-PCR-HRM) analysis was used to detect and discriminate four Babesia species infective to cattle, including Babesia bovis, B. bigemina, B. major and B. ovata. The melting profiles and melting temperatures (Tm) of the am

SUBMITTER: Wang J 

PROVIDER: S-EPMC6833191 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature