NHEJ pathway is involved in post-integrational DNA repair due to Ku70 binding to HIV-1 integrase.

ABSTRACT:

Background

HIV-1 integration results in genomic DNA gaps that are repaired by cellular DNA repair pathways. This step of the lentiviral life cycle remains poorly understood despite its crucial importance for successful replication. We and others reported that Ku70 protein of the non-homologous end joining pathway (NHEJ) directly binds HIV-1 integrase (IN). Here, we studied the importance of this interaction for post-integrational gap repair and the recruitment of NHEJ factors in this process.

Results

We engineered HIV-based pseudovirus with mutant IN defective in Ku70 binding and generated heterozygous Ku70, Ku80 and DNA-PKcs human knockout (KO) cells using CRISPR/Cas9. KO of either of these proteins or inhibition of DNA-PKcs catalytic activity substantially decreased the in

SUBMITTER: Knyazhanskaya E 

PROVIDER: S-EPMC6833283 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature