Quality control of imbalanced mass spectra from isotopic labeling experiments.

ABSTRACT: BACKGROUND:Mass spectra are usually acquired from the Liquid Chromatography-Mass Spectrometry (LC-MS) analysis for isotope labeled proteomics experiments. In such experiments, the mass profiles of labeled (heavy) and unlabeled (light) peptide pairs are represented by isotope clusters (2D or 3D) that provide valuable information about the studied biological samples in different conditions. The core task of quality control in quantitative LC-MS experiment is to filter out low-quality peptides with questionable profiles. The commonly used methods for this problem are the classification approaches. However, the data imbalance problems in previous control methods are often ignored or mishandled. In this study, we introduced a quality control framework based on the extreme gradient boosting mach

SUBMITTER: Li T 

PROVIDER: S-EPMC6833298 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature