Boosting Recombinant Inclusion Body Production-From Classical Fed-Batch Approach to Continuous Cultivation.

ABSTRACT: State of the art microbial recombinant protein production is regularly performed in fed-batch based cultivations. However, these cultivations suffer from highly time-dependent changes in productivity and product quality, leading to high variations in the downstream process. Continuous biomanufacturing offers the possibility of a time independent process, boosting the time-space-yield of the recombinantly produced protein and further reducing costs for production, also as downstream gets more predictive. In the current work, the continuous production of a pharmaceutically relevant protein in form of an inclusion body in E. coli BL21(DE3) was investigated in single vessel cultivations by varying dilution rates using glycerol as carbon source, inducer (lactose or IPTG) and respective i

SUBMITTER: Kopp J 

PROVIDER: S-EPMC6834550 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature