A functional genetic screen identifies the Mediator complex as essential for SSX2-induced senescence.

ABSTRACT: The senescence response to oncogenes is believed to be a barrier to oncogenic transformation in premalignant lesions, and describing the mechanisms by which tumor cells evade this response is important for early diagnosis and treatment. The male germ cell-associated protein SSX2 is ectopically expressed in many types of cancer and is functionally involved in regulating chromatin structure and supporting cell proliferation. Similar to many well-characterized oncogenes, SSX2 has the ability to induce senescence in cells. In this study, we performed a functional genetic screen to identify proteins implicated in SSX2-induced senescence and identified several subunits of the Mediator complex, which is central in regulating RNA polymerase-mediated transcription. Further experiments showed that r

SUBMITTER: Bruckmann NH 

PROVIDER: S-EPMC6834653 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature