Feasibility of Localized Metabolomics in the Study of Pancreatic Islets and Diabetes.

ABSTRACT: (1) Background: Disruption of insulin production by native or transplanted pancreatic islets caused by auto/allo-immunity leads to hyperglycemia, a serious health condition and important therapeutic challenge due to the lifelong need for exogeneous insulin administration. Early metabolic biomarkers can prompt timely interventions to preserve islet function, but reliable biomarkers are currently lacking. We explored the feasibility of "localized metabolomics" where initial biomarker discovery is made in aqueous humor samples for further validation in the circulation. (2) Methods: We conducted non-targeted metabolomic studies in parallel aqueous humor and plasma samples from diabetic and nondiabetic mice. Metabolite levels and associated pathways were compared in both compartments as well as

SUBMITTER: Alcazar O 

PROVIDER: S-EPMC6835460 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature