Stabilization of Deformable Nanovesicles Based on Insulin-Phospholipid Complex by Freeze-Drying.

ABSTRACT: Deformable nanovesicles have been extensively investigated due to their excellent ability to penetrate biological barriers. However, suffering from serious physical and chemical instabilities, the wide use of deformable nanovesicles in medical applications is still limited. Moreover, far less work has been done to pursue the lyophilization of deformable nanovesicles. Here, we aimed to obtain stable deformable nanovesicles via freeze-drying technology and to uncover the underlying protection mechanisms. Firstly, the density of nanovesicles before freeze-drying, the effect of different kinds of cryoprotectants, and the types of different reconstituted solvents after lyophilization were investigated in detail to obtain stable deformable nanovesicles based on insulin-phospholipid complex (IPC-

SUBMITTER: Xu Y 

PROVIDER: S-EPMC6835673 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature