Hepatic Metabolic Derangements Triggered by Hyperthermia: An In Vitro Metabolomic Study.

ABSTRACT:

Background and aims

Liver toxicity is a well-documented and potentially fatal adverse complication of hyperthermia. However, the impact of hyperthermia on the hepatic metabolome has hitherto not been investigated.

Methods

In this study, gas chromatography-mass spectrometry (GC-MS)-based metabolomics was applied to assess the in vitro metabolic response of primary mouse hepatocytes (PMH, n = 10) to a heat stress stimulus, i.e., after 24 h exposure to 40.5 °C. Metabolomic profiling of both intracellular metabolites and volatile metabolites in the extracellular medium of PMH was performed.

Results

Multivariate analysis showed alterations in levels of 22 intra- and 59 extracellular metabolites, unveiling the capability of the metabolic pattern to discriminate cells

SUBMITTER: Araujo AM 

PROVIDER: S-EPMC6835778 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature