Cytocompatible and Antibacterial Properties of Chitosan-Siloxane Hybrid Spheres.

ABSTRACT: Microporous spheres in a hybrid system consisting of chitosan and ?-glycidoxypropyltrimethoxysilane (GPTMS) have advantages in a range of applications, e.g., as vehicles for cell transplantation and soft tissue defect filling materials, because of their excellent cytocompatibility with various cells. In this study, microporous chitosan-GPTMS spheres were prepared by dropping chitosan-GPTMS precursor sols, with or without a cerium chloride, into liquid nitrogen using a syringe pump. The droplets were then freeze dried to give the pores of size 10 to 50 ?m. The cell culture tests showed that L929 fibroblast-like cells migrated into the micropores larger than 50 ?m in diameter, whereas MG63 osteoblast-like cells proliferated well and covered the granule surfaces. The spheres with cerium chloride showed antibacterial properties against both gram-negative and gram-positive bacteria.

SUBMITTER: Shirosaki Y 

PROVIDER: S-EPMC6835879 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature