Normalizing Untargeted Periconceptional Urinary Metabolomics Data: A Comparison of Approaches.

ABSTRACT: Metabolomics studies of the early-life exposome often use maternal urine specimens to investigate critical developmental windows, including the periconceptional period and early pregnancy. During these windows changes in kidney function can impact urine concentration. This makes accounting for differential urinary dilution across samples challenging. Because there is no consensus on the ideal normalization approach for urinary metabolomics data, this study's objective was to determine the optimal post-analytical normalization approach for untargeted metabolomics analysis from a periconceptional cohort of 45 women. Urine samples consisted of 90 paired pre- and post-implantation samples. After untargeted mass spectrometry-based metabolomics analysis, we systematically compared the performanc

SUBMITTER: Rosen Vollmar AK 

PROVIDER: S-EPMC6835889 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature