High-Throughput 16S rRNA Sequencing to Assess Potentially Active Bacteria and Foodborne Pathogens: A Case Example in Ready-to-Eat Food.

ABSTRACT: Technologies to detect the entire bacterial diversity spectra and foodborne pathogens in food represent a fundamental advantage in the control of foodborne illness. Here, we applied high-throughput 16S rRNA sequencing of amplicons obtained by PCR and RT-PCR from extracted DNA and RNA targeting the entire bacterial community and the active bacterial fraction present in some of the most consumed and distributed ready-to-eat (RTE) salad brands in Europe. Customer demands for RTE food are increasing worldwide along with the number of associated foodborne illness and outbreaks. The total aerobic bacterial count in the analyzed samples was in the range of 2-4 × 10⁶ CFU/g (SD ± 1.54 × 10⁶). Culture validated methods did not detect Salmonella spp., Escherichia coli<

SUBMITTER: Mira Miralles M 

PROVIDER: S-EPMC6836182 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature