Single-Dimer Formation Rate Reveals Heterogeneous Particle Surface Reactivity.

ABSTRACT: Biofunctionalized micro- and nanoparticles are important for a wide range of applications, but methodologies to measure, modulate, and model interactions between individual particles are scarce. Here, we describe a technique to measure the aggregation rate of two particles to a single dimer, by recording the trajectory that a particle follows on the surface of another particle as a function of time. The trajectory and the interparticle potential are controlled by a magnetic field. Particles were studied with and without conjugated antibodies in a wide range of pH conditions. The data shows that the aggregation process strongly depends on the particle surface charge density and hardly on the antibody surface coverage. Furthermore, microscopy videos of single particle dimers reveal the prese

SUBMITTER: Scheepers MRW 

PROVIDER: S-EPMC6836307 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature