Phenotypic and genotypic characterization of clinical Staphylococcus aureus isolates from Kenya.

ABSTRACT:

Background

The increase and spread of virulent-outbreak associated, methicillin and vancomycin resistant (MRSA/VRSA) Staphylococcus aureus require a better understanding of the resistance and virulence patterns of circulating and emerging strains globally. This study sought to establish the resistance phenotype, and strains of 32 non-duplicate clinical MRSA and MSSA S. aureus isolates from four Kenyan hospitals, identify their resistance and virulence genes and determine the genetic relationships of MRSA with global strains.

Methods

Antimicrobial susceptibility profiles were determined on a Vitek 2, genomic DNA sequenced on an Illumina Miseq and isolates typed in-silico. Resistance and virulence genes were identified using ARIBA and phylogenies generated using RAxML.

Resu

SUBMITTER: Kyany'a C 

PROVIDER: S-EPMC6836327 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature