Organelle genome composition and candidate gene identification for Nsa cytoplasmic male sterility in Brassica napus.

ABSTRACT:

Background

Nsa cytoplasmic male sterility (CMS) is a novel alloplasmic male sterility system derived from somatic hybridization between Brassica napus and Sinapis arvensis. Identification of the CMS-associated gene is a prerequisite for a better understanding of the origin and molecular mechanism of this CMS. With the development of genome sequencing technology, organelle genomes of Nsa CMS line and its maintainer line were sequenced by pyro-sequencing technology, and comparative analysis of the organelle genomes was carried out to characterize the organelle genome composition of Nsa CMS as well as to identify the candidate Nsa CMS-associated genes.

Results

Nsa CMS mitochondrial genome showed a higher collinearity with that of S. arvensis than B. napus, indicating that Nsa C

SUBMITTER: Sang SF 

PROVIDER: S-EPMC6836354 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature