Novel nose poke-based temporal discrimination tasks with concurrent in vivo calcium imaging in freely moving mice.

ABSTRACT: The hippocampus has been known to process temporal information as part of memory formation. While time cells have been observed in the hippocampus and medial entorhinal cortex, a number of the behavioral tasks used present potential confounds that may cause some contamination of time cell observations due to animal movement. Here, we report the development of a novel nose poke-based temporal discrimination task designed to be used with in vivo calcium imaging for the analysis of hippocampal time cells in freely moving mice. First, we developed a ten second held nose poke paradigm for use in mice to deliver a purer time metric for the analysis of time cell activity in hippocampus CA1. Second, we developed a temporal discrimination task that involves the association of held nose poke duratio

SUBMITTER: Marks WD 

PROVIDER: S-EPMC6836444 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature