Biochemical characterization and chemical validation of Leishmania MAP Kinase-3 as a potential drug target.

ABSTRACT: Protozoan parasites of the Leishmania genus have evolved unique signaling pathways that can sense various environmental changes and trigger stage differentiation for survival and host infectivity. MAP kinase (MAPK) plays a critical role in various cellular activities like cell differentiation, proliferation, stress regulation, and apoptosis. The Leishmania donovani MAPK3 (LdMAPK3) is involved in the regulation of flagella length and hence plays an important role in disease transmission. Here, we reported the gene cloning, protein expression, biochemical characterizations, inhibition studies and cell proliferation assay of LdMAPK3. The recombinant purified LdMAPK3 enzyme obeys the Michaelis-Menten equation with K_m and V_max of LdMAPK3 was found to be 20.23 nM and 38.77 

SUBMITTER: Raj S 

PROVIDER: S-EPMC6838069 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature