Direct interaction between the hepatitis B virus core and envelope proteins analyzed in a cellular context.

ABSTRACT: Hepatitis B virus (HBV) production requires intricate interactions between the envelope and core proteins. Analyses of mutants of these proteins have made it possible to map regions involved in the formation and secretion of virions. Tests of binding between core and envelope peptides have also been performed in cell-free conditions, to study the interactions potentially underlying these mechanisms. We investigated the residues essential for core-envelope interaction in a cellular context in more detail, by transiently producing mutant or wild-type L, S, or core proteins separately or in combination, in Huh7 cells. The colocalization and interaction of these proteins were studied by confocal microscopy and co-immunoprecipitation, respectively. The L protein was shown to constitute a molecu

SUBMITTER: Pastor F 

PROVIDER: S-EPMC6838148 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature