Genetic Ablation of Calcium-independent Phospholipase A₂γ Exacerbates Glomerular Injury in Adriamycin Nephrosis in Mice.

ABSTRACT: Genetic ablation of calcium-independent phospholipase A₂γ (iPLA₂γ) in mice results in marked damage of mitochondria and enhanced autophagy in glomerular visceral epithelial cells (GECs) or podocytes. The present study addresses the role of iPLA₂γ in glomerular injury. In adriamycin nephrosis, deletion of iPLA₂γ exacerbated albuminuria and reduced podocyte number. Glomerular LC3-II increased and p62 decreased in adriamycin-treated iPLA₂γ knockout (KO) mice, compared with treated control, in keeping with increased autophagy in KO. iPLA₂γ KO GECs in culture also demonstrated increased autophagy, compared with control GECs. iPLA₂γ KO GECs showed a reduced oxygen consumption rate and increased phosphorylation of AMP ki

SUBMITTER: Elimam H 

PROVIDER: S-EPMC6838178 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature