TGFβ induces stemness through non-canonical AKT-FOXO3a axis in oral squamous cell carcinoma.

ABSTRACT: BACKGROUND:FOXO3a has been widely regarded as a tumor suppressor. It also plays a paradoxical role in regulating the cancer stem cells (CSCs), responsible for tumor-initiation, chemo-resistance, and recurrence in various solid tumors, including oral squamous cell carcinoma (OSCC). This study aims to uncover the role of FOXO3a and its importance for a non-canonical pathway of TGFβ in regulating the OSCC stemness. METHODS:We identified FOXO3a expression in OSCC tissues and cell lines using immunohistochemistry and western blot. The correlation between FOXO3a and stemness was evaluated. Stable cell lines with differential expression of FOXO3a were constructed using lentiviruses. The effects of FOXO3a on stem-cell like properties in OSCC was further evaluated in vitro and in vivo. We also expl

SUBMITTER: Li K 

PROVIDER: S-EPMC6838363 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature