Transcriptome profiling reveals functional variation in Plasmodium falciparum parasites from controlled human malaria infection studies.

ABSTRACT:

Background

The transcriptome of Plasmodium falciparum clinical isolates varies according to strain, mosquito bites, disease severity and clinical history. Therefore, it remains a challenge to directly interpret the parasite's transcriptomic information into a more general biological signature in a natural human malaria infection. These confounding variations can be potentially overcome with parasites derived from controlled-human malaria infection (CHMI) studies.

Methods

We performed CHMI studies in healthy and immunologically naïve volunteers receiving the same P. falciparum strain ((Sanaria® PfSPZ Challenge (NF54)), but with different sporozoite dosage and route of infection. Parasites isolated from these volunteers at the day of patency were subjected to in vitro culture

SUBMITTER: Hoo R 

PROVIDER: S-EPMC6838377 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature