A single faecal microbiota transplantation modulates the microbiome and improves clinical manifestations in a rat model of colitis.

ABSTRACT: BACKGROUND:Faecal microbiota transplantation (FMT) is a novel potential therapy for inflammatory bowel diseases, but it is poorly characterised. METHODS:We evaluated the performance of the mouse and rat as a pre-clinical model for human microbiota engraftment. We then characterised the effect of a single human stool transfer (HST) on a humanised model of DSS-induced colitis. Colonic and faecal microbial communities were analysed using the 16S rRNA approach and clinical manifestations were assessed in a longitudinal setting. FINDINGS:The microbial community of rats showed greater similarity to that of humans, while the microbiome of mice showed less similarity to that of humans. Moreover, rats captured more human microbial species than mice after a single HST. Using the rat model, we showed

SUBMITTER: Lleal M 

PROVIDER: S-EPMC6838378 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature