Accumulation of mitochondrial 7S DNA in idiopathic and LRRK2 associated Parkinson's disease.

ABSTRACT:

Background

Both idiopathic and familial Parkinson's disease are associated with mitochondrial dysfunction. Mitochondria have their own mitochondrial DNA (mtDNA) and previous studies have reported that the release of mtDNA is a biomarker of Parkinson's disease.

Methods

We have now investigated the relationship between mtDNA replication, transcription and release in fibroblasts from patients with idiopathic (iPD) and Leucine-rich repeat kinase 2^G2019S -associated Parkinson's disease (LRRK2-PD), using Selfie-digital PCR, a method that allows absolute quantification of mtDNA genomes and transcripts.

Findings

In comparison with healthy controls, we found that fibroblasts from patients with iPD or LRRK2-PD had a high amount of mitochondrial 7S DNA along with a lo

SUBMITTER: Podlesniy P 

PROVIDER: S-EPMC6838390 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature