Dual function of miR-1248 links interferon induction and calcium signaling defects in Sjogren's syndrome.

ABSTRACT:

Background

Sjögren's syndrome (SS) is one of the most common autoimmune disorders leading to exocrine gland dysfunction. Both immune-dependent processes - like Type I Interferon (IFN) signaling and immune-independent processes - such as calcium signaling in epithelial cells - contribute to disease pathophysiology. However, a mechanistic link between these processes has not been demonstrated.

Methods

Primary human salivary gland cells were used to evaluate the differential expression of miRNAs with smRNA-seq in primary epithelial cells culture and digital PCR was conducted in SS human salivary glands (SG) biopsies to verify the results. With siRNA screening and pull-down assays to establish the role of miRNA in IFN activation.

Findings

Activation of IFN-β by miR-1248 i

SUBMITTER: Jang SI 

PROVIDER: S-EPMC6838412 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature