Overexpression of Limb-Bud and Heart (LBH) promotes angiogenesis in human glioma via VEGFA-mediated ERK signalling under hypoxia.

ABSTRACT: BACKGROUND:Glioma is the most common primary malignant tumor in the central nervous system with frequent hypoxia and angiogenesis. Limb-Bud and Heart (LBH) is a highly conserved transcription cofactor that participates in embryonic development and tumorigenesis. METHODS:The conditioned media from LBH regulated human glioma cell lines and patient-derived glioma stem cells (GSCs) were used to treat the human brain microvessel endothelial cells (hBMECs). The function of LBH on angiogenesis were examined through methods of MTS assay, Edu assay, TUNEL assay, western blotting analysis, qPCR analysis, luciferase reporter assay and xenograft experiment. FINDINGS:Our study found for the first time that LBH was overexpressed in gliomas and was associated with a poor prognosis. LBH overexpression par

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC6838451 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature