Prevalence of Virulence Genes in Enteroaggregative Escherichia coli Isolates from Young Children from Rural South Africa.

ABSTRACT: In this study, we report on the prevalence of 19 virulence genes in enteroaggregative Escherichia coli (EAEC) isolates from northern South Africa. Stool samples obtained prospectively from 97 children from 1 to 12 months of age were analyzed, and EAEC isolates were confirmed based on the presence of aaiC or aatA genes. We investigated 177 enteroaggregative Escherichia coli isolates for the prevalence of virulence genes using multiplex polymerase chain reaction. The chromosomal gene aaiC was detected at higher frequency (48.0%) compared with aatA (26.0%). The gene encoding the open reading frame Orf61 was the most prevalent putative virulence trait detected among the isolates (150/177; 84.7%). None of the genes was statistically associated wi

SUBMITTER: Tanih NF 

PROVIDER: S-EPMC6838558 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature