Decreased Enhancer-Promoter Proximity Accompanying Enhancer Activation.

ABSTRACT: Enhancers can regulate the promoters of their target genes over very large genomic distances. It is widely assumed that mechanisms of enhancer action involve the reorganization of three-dimensional chromatin architecture, but this is poorly understood. The predominant model involves physical enhancer-promoter interaction by looping out the intervening chromatin. However, studying the enhancer-driven activation of the Sonic hedgehog gene (Shh), we have identified a change in chromosome conformation that is incompatible with this simple looping model. Using super-resolution 3D-FISH and chromosome conformation capture, we observe a decreased spatial proximity between Shh and its enhancers during the differentiation of embryonic stem cells to neural progenitors. We show that this can be recapi

SUBMITTER: Benabdallah NS 

PROVIDER: S-EPMC6838673 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature