Multiplex detection of six swine viruses on an integrated centrifugal disk using loop-mediated isothermal amplification.

ABSTRACT: Advances in molecular testing and microfluidic technologies have opened new avenues for rapid detection of animal viruses. We used a centrifugal microfluidic disk (CMFD) to detect 6 important swine viruses, including foot-and-mouth disease virus, classical swine fever virus, porcine reproductive and respiratory swine virus-North American genotype, porcine circovirus 2, pseudorabies virus, and porcine parvovirus. Through integrating the loop-mediated isothermal amplification (LAMP) method and microfluidic chip technology, the CMFD could be successfully performed at 62℃ in 60 min. The detection limit of the CMFD was 3.2 × 10² copies per reaction, close to the sensitivity of tube-type LAMP turbidity methods (1 × 10² copies per reaction). In addition, the CMFD was highly

SUBMITTER: Yuan X 

PROVIDER: S-EPMC6838697 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature