Two-Color Spatial Cumulant Analysis Detects Heteromeric Interactions between Membrane Proteins.

ABSTRACT: Fluorescence fluctuation spectroscopy can be used to measure the aggregation of fluorescently labeled molecules and is typically performed using time series data. Spatial intensity distribution analysis and fluorescence moment image analysis are established tools for measuring molecular brightnesses from single-color images collected with laser scanning microscopes. We have extended these tools for analysis of two-color images to resolve heteromeric interactions between molecules labeled with spectrally distinct chromophores. We call these new methods two-color spatial intensity distribution analysis and two-color spatial cumulant analysis (2c-SpCA). To implement these techniques on a hyperspectral imaging system, we developed a spectral shift filtering technique to remove artifacts due to

SUBMITTER: Foust DJ 

PROVIDER: S-EPMC6838761 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature