High production of valencene in Saccharomyces cerevisiae through metabolic engineering.

ABSTRACT:

Background

The biological synthesis of high value compounds in industry through metabolically engineered microorganism factories has received increasing attention in recent years. Valencene is a high value ingredient in the flavor and fragrance industry, but the low concentration in nature and high cost of extraction limits its application. Saccharomyces cerevisiae, generally recognized as safe, is one of the most commonly used gene expression hosts. Construction of S. cerevisiae cell factory to achieve high production of valencene will be attractive.

Results

Valencene was successfully biosynthesized after introducing valencene synthase into S. cerevisiae BJ5464. A significant increase in valencene yield was observed after down-regulation or knock-out of squalene synthesis a

SUBMITTER: Chen H 

PROVIDER: S-EPMC6839068 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature