Template-switching artifacts resemble alternative polyadenylation.

ABSTRACT:

Background

Alternative polyadenylation is commonly examined using cDNA sequencing, which is known to be affected by template-switching artifacts. However, the effects of such template-switching artifacts on alternative polyadenylation are generally disregarded, while alternative polyadenylation artifacts are attributed to internal priming.

Results

Here, we analyzed both long-read cDNA sequencing and direct RNA sequencing data of two organisms, generated by different sequencing platforms. We developed a filtering algorithm which takes into consideration that template-switching can be a source of artifactual polyadenylation when filtering out spurious polyadenylation sites. The algorithm outperformed the conventional internal priming filters based on comparison to direct RNA s

SUBMITTER: Balazs Z 

PROVIDER: S-EPMC6839120 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature