Fluorescent probes towards selective cathepsin B detection and visualization in cancer cells and patient samples.

ABSTRACT: Human cysteine cathepsins constitute an 11-membered family of proteases responsible for degradation of proteins in cellular endosomal-lysosomal compartments as such, they play important roles in antigen processing, cellular stress signaling, autophagy, and senescence. Moreover, for many years these enzymes were also linked to tumor growth, invasion, angiogenesis and metastasis when upregulated. Individual biological roles of each cathepsin are difficult to establish, because of their redundancy and similar substrate specificities. Selective chemical tools that enable imaging of individual cathepsin activities in living cells, tumors, and the tumor microenvironment may provide a better insight into their functions. In this work, we used HyCoSuL technology to profile the substrate specificit

SUBMITTER: Poreba M 

PROVIDER: S-EPMC6839509 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature