An open-space microfluidic chip with fluid walls for online detection of VEGF via rolling circle amplification.

ABSTRACT: Despite traditional poly-dimethyl siloxane (PDMS) microfluidic devices having great potential in various biological studies, they are limited by sophisticated fabrication processes and low utilization. An easily controlled microfluidic platform with high efficiency and low cost is desperately required. In this work, we present an open-space microfluidic chip with fluid walls, integrating cell culture and online semi-quantitative detection of vascular endothelial growth factor (VEGF) via rolling circle amplification (RCA) reaction. In comparison with conventional co-culture detecting platforms, this method features the prominent advantages of saving reagents and time, a simplified chip fabrication process, and avoiding additional assistance for online detection with the help of an interfaci

SUBMITTER: Feng S 

PROVIDER: S-EPMC6839512 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature